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Anti-HNRNPA1 Antibody
Catalog #: 144-07491
HNRNPA1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P09651 |
| Gene Id | 3178 |
| Gene Symbols | HNRNPA1 |
| Protein Name / Synonyms | HNRNPA1, ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, UP 1, hnRNP A1, hnRNP-A1, hnRNPA1, UP1, heterogeneous nuclear ribonucleoprotein A1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
29kDa/34kDa/38kDa
Observed MW
39kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-372 of human HNRNPA1 (NP_112420.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HNRNPA1 antibody (144-07491) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min.
Immunohistochemistry of paraffin-embedded human gastric injury using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spinal cord using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain astrocytoma using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA1 Antibody (144-07491) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using HNRNPA1 antibody (144-07491).
Expiration:
12 months from the date of shipment when stored properly.