Anti-HNRNPA2B1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P22626
Gene Id	3181
Gene Symbols	HNRNPA2B1
Protein Name / Synonyms	HNRNPA2B1, HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, IBMPFD2, RNPA2, SNRPB1, heterogeneous nuclear ribonucleoprotein A2/B1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

36kDa/37kDa

Observed MW

37kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA2B1 (NP_002128.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HNRNPA2B1 antibody (144-01162) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Western blot analysis of extracts from normal (control) and HNRNPA2B1 knockout (KO) 293T cells, using HNRNPA2B1 antibody (144-01162) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Immunohistochemistry of paraffin-embedded human colon using HNRNPA2B1 Antibody (144-01162) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Immunohistochemistry of paraffin-embedded human kidney using HNRNPA2B1 Antibody (144-01162) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Immunohistochemistry of paraffin-embedded human stomach using HNRNPA2B1 Antibody (144-01162) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Immunohistochemistry of paraffin-embedded mouse brain using HNRNPA2B1 Antibody (144-01162) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA2B1 Antibody (144-01162) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Immunohistochemistry of paraffin-embedded rat brain using HNRNPA2B1 Antibody (144-01162) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Immunohistochemistry of paraffin-embedded rat kidney using HNRNPA2B1 Antibody (144-01162) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Immunohistochemistry of paraffin-embedded rat liver using HNRNPA2B1 Antibody (144-01162) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Immunohistochemistry of paraffin-embedded human kidney cancer using HNRNPA2B1 Antibody (144-01162) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Immunohistochemistry of paraffin-embedded human gastric cancer using HNRNPA2B1 Antibody (144-01162) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Immunofluorescence analysis of HeLa cells using HNRNPA2B1 antibody (144-01162). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] HNRNPA2B1 Polyclonal Antibody (144-01162)

Expiration:

12 months from the date of shipment when stored properly.

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