3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-HNRNPD Antibody
Catalog #: 144-01828
HNRNPD Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q14103 |
| Gene Id | 3184 |
| Gene Symbols | HNRNPD |
| Protein Name / Synonyms | HNRNPD, AUF1, AUF1A, HNRPD, P37, hnRNPD0, heterogeneous nuclear ribonucleoprotein D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
30kDa/32kDa/36kDa/38kDa
Observed MW
43kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of human HNRNPD (NP_002129.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HNRNPD antibody (144-01828) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human adenoma of uterine muscle using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using HNRNPD Antibody (144-01828) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HNRNPD antibody (144-01828).
Expiration:
12 months from the date of shipment when stored properly.