Anti-HNRNPD (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q14103
Gene Id	3184
Gene Symbols	HNRNPD
Protein Name / Synonyms	HNRNPD (N-term)
Clonality	Polyclonal
Preparation	This HNRNPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-85 amino acids from the N-terminal region of human HNRNPD.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

HNRNPD; AUF1; HNRPD; Heterogeneous nuclear ribonucleoprotein D0; AU-rich element RNA-binding protein 1

NCBI Accession #

NP_001003810.1;NP_002129.2;NP_112737.1;NP_112738.1

Other Accession #

Q9JJ54; Q60668; NP_002129.2; NP_112738.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

38434

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

HNRNPD Antibody (N-term) (Cat# 102-12849) western blot analysis in 293T,A431,NCI-H292,Raji cell line lysates (35ug/lane).This demonstrates the HNRNPD antibody detected the HNRNPD protein (arrow).

Fluorescent confocal image of Hela cell stained with HNRNPD Antibody (N-term)(Cat# 102-12849).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRNPD primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRNPD immunoreactivity is localized to Nucleus and Cytoplasm significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products