Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P61978 |
| Gene Id | 3190 |
| Gene Symbols | HNRNPK |
| Protein Name / Synonyms | HNRNPK, AUKS, CSBP, HNRPK, TUNP, heterogeneous nuclear ribonucleoprotein K |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
48kDa/50kDa/51kDa
Observed MW
60kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPK (NP_112552.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HNRNPK antibody (144-01701) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat lung using HNRNPK Antibody (144-01701) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using HNRNPK Antibody (144-01701) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HNRNPK Antibody (144-01701) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using HNRNPK Antibody (144-01701) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using HNRNPK Antibody (144-01701) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HNRNPK Antibody (144-01701) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using HNRNPK Antibody (144-01701) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse esophagus using HNRNPK Antibody (144-01701) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HNRNPK Antibody (144-01701) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using HNRNPK antibody (144-01701). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.