HNRNPM Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P52272 |
| Gene Id | 4670 |
| Gene Symbols | HNRNPM |
| Protein Name / Synonyms | HNRNPM, CEAR, HNRNPM4, HNRPM, HNRPM4, HTGR1, NAGR1, hnRNP M, hnRNPM, heterogeneous nuclear ribonucleoprotein M |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
73kDa/77kDa
Observed MW
78kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 491-730 of human HNRNPM (NP_005959.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HNRNPM antibody (144-06937) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using HNRNPM antibody (144-06937) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HNRNPM antibody (144-06937) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using HNRNPM antibody (144-06937) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HNRNPM antibody (144-06937) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPM antibody (144-06937) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HNRNPM antibody (144-06937) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using HNRNPM antibody (144-06937).
Expiration:
12 months from the date of shipment when stored properly.