Anti-HNRPAB (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q99729
Gene Id	3182
Gene Symbols	HNRNPAB
Protein Name / Synonyms	HNRPAB (N-term)
Clonality	Polyclonal
Preparation	This HNRPAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HNRPAB.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs.

Specificity

Other Names

HNRNPAB; ABBP1; HNRPAB; Heterogeneous nuclear ribonucleoprotein A/B; APOBEC1-binding protein 1

NCBI Accession #

NP_004490.2;NP_112556.2

Other Accession #

NP_112556

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

36225

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50

	HNRPAB Antibody (N-term) (Cat# 102-16522) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HNRPAB antibody detected the HNRPAB protein (arrow).
	HNRPAB Antibody (N-term) (Cat# 102-16522) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HNRPAB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Fluorescent confocal image of MCF-7 cell stained with HNRPAB Antibody (N-term)(Cat# 102-16522).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRPAB primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRPAB immunoreactivity is localized to Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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