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Anti-HPD Antibody
Catalog #: 144-06505
HPD Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P32754 |
| Gene Id | 3242 |
| Gene Symbols | HPD |
| Protein Name / Synonyms | HPD, 4-HPPD, 4HPPD, GLOD3, HPPDASE, PPD, 4-hydroxyphenylpyruvate dioxygenase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
40kDa/44kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human HPD (NP_002141.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HPD antibody (144-06505) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat kidney using HPD antibody (144-06505) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HPD antibody (144-06505) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HPD antibody (144-06505). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.