Anti-HPSE Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9Y251
Gene Id	10855
Gene Symbols	HPSE
Protein Name / Synonyms	HPSE, HPA, HPA1, HPR1, HPSE1, HSE1, heparanase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:10 - 1:100

Format

Calculated MW

42kDa/53kDa/54kDa/61kDa

Observed MW

54kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 36-275 of human HPSE (NP_001092010.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HPSE antibody (144-05726) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - HPSE Polyclonal Antibody (144-05726)

Immunofluorescence analysis of U2OS cells using HPSE antibody (144-05726). Blue: DAPI for nuclear staining.
Immunofluorescence - HPSE Polyclonal Antibody (144-05726)

Expiration:

12 months from the date of shipment when stored properly.

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