RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-HS1BP3 Antibody

Catalog #: 144-08250

HS1BP3 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q53T59
Gene Id
64342
Gene Symbols
HS1BP3
Protein Name / Synonyms
HS1BP3, ETM2, HS1-BP3, HCLS1-binding protein 3
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene shares similarity with mouse Hs1bp3, an Hcls1/Hs1-interacting protein that may be involved in lymphocyte activation.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100

Format

Calculated MW
42kDa
Observed MW
55kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HS1BP3 (NP_071905.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HS1BP3 antibody (144-08250) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - HS1BP3 antibody (144-08250)

Immunohistochemistry of paraffin-embedded human stomach using HS1BP3 antibody (144-08250) at dilution of 1:100 (40x lens).
Immunohistochemistry - HS1BP3 antibody (144-08250)

Immunohistochemistry of paraffin-embedded rat kidney using HS1BP3 antibody (144-08250) at dilution of 1:100 (40x lens).
Immunohistochemistry - HS1BP3 antibody (144-08250)

Immunofluorescence analysis of U2OS cells using HS1BP3 antibody (144-08250) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - HS1BP3 antibody (144-08250)

Expiration:
12 months from the date of shipment when stored properly.