Anti-HSP27 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04792
Gene Id	3315
Gene Symbols	HSPB1
Protein Name / Synonyms	HSP27 (Center)
Clonality	Polyclonal
Preparation	This HSP27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-136 amino acids from the Central region of human HSP27.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Angiogenesis, Cancer, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

HSPB1; HSP27; HSP28; Heat shock protein beta-1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; Stress-responsive protein 27

NCBI Accession #

NP_001531.1

Other Accession #

P42930; P14602; Q3T149

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

22783

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of HSP27 antibody (Center) (Cat# 102-17175) in MCF-7 cell line lysates (35ug/lane). HSP27 (arrow) was detected using the purified Pab.
	Hsp 27 Antibody (Center) (Cat# 102-17175) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Hsp 27 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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