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Anti-HSP70 Antibody
Catalog #: 130-10052
Rabbit Anti-Human HSP70 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P08107 |
| Gene Id | 3303, 3304 |
| Gene Symbols | HSPA1A HSP72 HSPA1 HSX70 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human HSP70. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CNPIISGLYQGAGGPGPGGFGAQGPK |
| Recommended Applications | ELISA (recommended work dilution= 1: 20, 000(at least detecting 50 ng/ml)) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat HSP70 has not yet been tested.
Storage
Keep it at 4°C if intended for use within a month. Store at -20°C if over months. Minimize freezing and thawing when use.
Related Products
Mouse Anti-Mycobacterium tuberculosis Heat Shock Protein-70 (HSP70) (cat# MD-02-0038 ) Mouse Anti-Human Heat Shock Protein-70 (HSP70) (cat# MD-02-0037 )
References
- Mayer MP, et al..(2005) Hsp70 chaperones: cellular functions and molecular mechanism. Cell Mol Life Sci. 62(6):670-84.
- Petrof EO, Ciancio MJ, Chang EB. Role and regulation of intestinal epithelial heat shock proteins in health and disease. Chin J Dig Dis. 2004;5(2):45-50.
Expiration:
12 months from the date of shipment when stored properly.