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Anti-HSP70 Antibody, Biotinylated
Catalog #: 130-10052B-50
Rabbit anti-HSP70 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P08107 |
| Gene Id | 3303, 3304 |
| Gene Symbols | HSPA1A HSP72 HSPA1 HSX70 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human HSP70. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CNPIISGLYQGAGGPGPGGFGAQGPK |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 20, 000(at least detecting 50 µg/ml)) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat HSP70 has not yet been tested.
Related Products
Mouse Anti-Mycobacterium tuberculosis Heat Shock Protein-70 (HSP70) (cat# MD-02-0038 ) Mouse Anti-Human Heat Shock Protein-70 (HSP70) (cat# MD-02-0037 )
References
- Mayer MP, et al..(2005) Hsp70 chaperones: cellular functions and molecular mechanism. Cell Mol Life Sci. 62(6):670-84.
- Petrof EO, Ciancio MJ, Chang EB. Role and regulation of intestinal epithelial heat shock proteins in health and disease. Chin J Dig Dis. 2004;5(2):45-50.
Expiration:
12 months from the date of shipment when stored properly.