RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-HSP90AB1 Antibody

Catalog #: 144-01087

HSP90AB1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P08238
Gene Id
3326
Gene Symbols
HSP90AB1
Protein Name / Synonyms
HSP90AB1, D6S182, HSP84, HSP90B, HSPC2, HSPCB, heat shock protein HSP 90-beta
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
83kDa
Observed MW
80kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human HSP90AB1 (NP_031381.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HSP90AB1 antibody (144-01087) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - HSP90AB1 antibody (144-01087)

Immunohistochemistry of paraffin-embedded rat intestine using HSP90AB1 Antibody (144-01087) at dilution of 1:100 (40x lens).
Immunohistochemistry - HSP90AB1 Antibody (144-01087)

Immunohistochemistry of paraffin-embedded rat lung using HSP90AB1 Antibody (144-01087) at dilution of 1:100 (40x lens).
Immunohistochemistry - HSP90AB1 Antibody (144-01087)

Immunohistochemistry of paraffin-embedded rat kidney using HSP90AB1 Antibody (144-01087) at dilution of 1:100 (40x lens).
Immunohistochemistry - HSP90AB1 Antibody (144-01087)

Immunohistochemistry of paraffin-embedded human colon using HSP90AB1 Antibody (144-01087) at dilution of 1:100 (40x lens).
Immunohistochemistry - HSP90AB1 Antibody (144-01087)

Immunohistochemistry of paraffin-embedded human esophagus using HSP90AB1 Antibody (144-01087) at dilution of 1:100 (40x lens).
Immunohistochemistry - HSP90AB1 Antibody (144-01087)

Immunohistochemistry of paraffin-embedded mouse lung using HSP90AB1 Antibody (144-01087) at dilution of 1:100 (40x lens).
Immunohistochemistry - HSP90AB1 Antibody (144-01087)

Immunohistochemistry of paraffin-embedded mouse kidney using HSP90AB1 Antibody (144-01087) at dilution of 1:100 (40x lens).
Immunohistochemistry - HSP90AB1 Antibody (144-01087)

Immunofluorescence analysis of U2OS cells using HSP90AB1 antibody (144-01087). Blue: DAPI for nuclear staining.
Immunofluorescence - HSP90AB1 antibody (144-01087)

Expiration:
12 months from the date of shipment when stored properly.