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Anti-HSP90B1 Antibody

Catalog #: 144-00989

HSP90B1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P14625
Gene Id
7184
Gene Symbols
HSP90B1
Protein Name / Synonyms
HSP90B1, ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1, endoplasmin
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
92kDa
Observed MW
110kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HSP90B1 (NP_003290.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HSP90B1 antibody (144-00989) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - HSP90B1 Polyclonal Antibody (144-00989)

Immunofluorescence analysis of HeLa cells using HSP90B1 antibody (144-00989) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - HSP90B1 Polyclonal Antibody (144-00989)

Immunofluorescence analysis of NIH/3T3 cells using HSP90B1 antibody (144-00989) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - HSP90B1 Polyclonal Antibody (144-00989)

Immunofluorescence analysis of PC-12 cells using HSP90B1 antibody (144-00989) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - HSP90B1 Polyclonal Antibody (144-00989)

Expiration:
12 months from the date of shipment when stored properly.