Anti-HSP90B1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Hamster
Host Species	Rabbit
Accession Number	P14625
Gene Id	7184
Gene Symbols	HSP90B1
Protein Name / Synonyms	HSP90B1 (N-term)
Clonality	Polyclonal
Preparation	This HSP90B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-43 amino acids from the N-terminal region of human HSP90B1.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

HSP90B1 is highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol.

Specificity

Predicted Reactivity

Rat, Bovine, Chicken, Pig, Monkey

Other Names

HSP90B1; GRP94; TRA1; Endoplasmin; 94 kDa glucose-regulated protein; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog

NCBI Accession #

NP_003290.1

Other Accession #

Q66HD0; Q29092; P08113; Q4R520; P08110; Q95M18

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

92469

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	HSP90B1 Antibody (N-term) (Cat# 102-15789) western blot analysis in HepG2, CHO, K562, Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the HSP90B1 antibody detected the HSP90B1 protein (arrow).
	HSP90B1 Antibody (N-term) (Cat# 102-15789) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSP90B1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	HSP90B1 Antibody (N-term) (Cat# 102-15789) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Confocal immunofluorescent analysis of HSP90B1 Antibody (N-term)(Cat# 102-15789) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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