RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-HSPA14 Antibody

Catalog #: 144-07107

HSPA14 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q0VDF9
Gene Id
51182
Gene Symbols
HSPA14
Protein Name / Synonyms
HSPA14, HSP70-4, HSP70L1, heat shock 70 kDa protein 14
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW
54kDa
Observed MW
55kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 240-509 of human HSPA14 (NP_057383.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HSPA14 antibody (144-07107) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - HSPA14 antibody (144-07107)

Immunofluorescence analysis of A549 cells using HSPA14 antibody (144-07107). Blue: DAPI for nuclear staining.
Immunofluorescence - HSPA14 antibody (144-07107)

Expiration:
12 months from the date of shipment when stored properly.