RayBiotech

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ISO 13485:2016 cGMP

Anti-HSPA1L Antibody

Catalog #: 144-01856

HSPA1L Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P34931
Gene Id
3305
Gene Symbols
HSPA1L
Protein Name / Synonyms
HSPA1L, HSP70-1L, HSP70-HOM, HSP70T, hum70t, heat shock 70 kDa protein 1-like
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
70kDa
Observed MW
67kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 342-641 of human HSPA1L (NP_005518.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HSPA1L antibody (144-01856) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - HSPA1L antibody (144-01856)

Immunofluorescence analysis of U2OS cells using HSPA1L antibody (144-01856). Blue: DAPI for nuclear staining.
Immunofluorescence - HSPA1L antibody (144-01856)

Expiration:
12 months from the date of shipment when stored properly.