Anti-HSPA5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P11021
Gene Id	3309
Gene Symbols	HSPA5
Protein Name / Synonyms	HSPA5, BIP, GRP78, HEL-S-89n, MIF2, 78 kDa glucose-regulated protein
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

72kDa

Observed MW

78kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HSPA5 (NP_005338.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HSPA5 antibody (144-00241) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - HSPA5 Polyclonal Antibody (144-00241)

Immunohistochemistry of paraffin-embedded rat brain using HSPA5 antibody (144-00241) at dilution of 1:100 (40x lens).
Immunohistochemistry - HSPA5 Polyclonal Antibody (144-00241)

Expiration:

12 months from the date of shipment when stored properly.

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