Anti-HSPB2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q16082
Gene Id	3316
Gene Symbols	HSPB2
Protein Name / Synonyms	HSPB2, HSP27, Hs.78846, LOH11CR1K, MKBP, heat shock protein beta-2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The protein is expressed preferentially in the heart and skeletal muscle. This protein regulates Myotonic Dystrophy Protein Kinase, which plays an important role in maintenance of muscle structure and function.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

20kDa

Observed MW

18kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human HSPB2 (NP_001532.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HSPB2 antibody (144-02350) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - HSPB2 antibody (144-02350)

Immunohistochemistry of paraffin-embedded mouse heart using HSPB2 antibody (144-02350) at dilution of 1:200 (40x lens).
Immunohistochemistry - HSPB2 antibody (144-02350)

Immunofluorescence analysis of U2OS cells using HSPB2 antibody (144-02350). Blue: DAPI for nuclear staining.
Immunofluorescence - HSPB2 antibody (144-02350)

Expiration:

12 months from the date of shipment when stored properly.

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