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Anti-HSPB8 Antibody
Catalog #: 144-02514
HSPB8 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UJY1 |
| Gene Id | 26353 |
| Gene Symbols | HSPB8 |
| Protein Name / Synonyms | HSPB8, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22, heat shock protein beta-8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
21kDa
Observed MW
22kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HSPB8 antibody (144-02514) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using HSPB8 antibody (144-02514) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using HSPB8 antibody (144-02514) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using HSPB8 antibody (144-02514) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human normal colon using HSPB8 antibody (144-02514) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon cancer using HSPB8 antibody (144-02514) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HSPB8 antibody (144-02514) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using HSPB8 antibody (144-02514) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using HSPB8 antibody (144-02514). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.