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Anti-HSPH1 Antibody
Catalog #: 144-06622
HSPH1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q92598 |
| Gene Id | 10808 |
| Gene Symbols | HSPH1 |
| Protein Name / Synonyms | HSPH1, HSP105, HSP105A, HSP105B, NY-CO-25, heat shock protein 105 kDa |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
92kDa/96kDa/97kDa
Observed MW
120kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 659-858 of human HSPH1 (NP_006635.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HSPH1 antibody (144-06622) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat spleen using HSPH1 antibody (144-06622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat fallopian tube using HSPH1 antibody (144-06622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using HSPH1 antibody (144-06622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using HSPH1 antibody (144-06622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using HSPH1 antibody (144-06622) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HSPH1 antibody (144-06622) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.