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Anti-HTR3A Antibody
Catalog #: 144-05647
HTR3A Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P46098 |
| Gene Id | 3359 |
| Gene Symbols | HTR3A |
| Protein Name / Synonyms | HTR3A, 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3, 5-hydroxytryptamine receptor 3A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Neuroscience |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
53kDa/55kDa/58kDa/59kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 24-241 of human HTR3A (NP_000860.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HTR3A antibody (144-05647) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using HTR3A antibody (144-05647) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HTR3A antibody (144-05647) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.