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Anti-HTRA2/Omi Antibody
Catalog #: 130-10034
Rabbit Anti-Human HTRA2/Omi Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | O43464 |
| Gene Id | 27429 |
| Gene Symbols | HTRA2 OMI PRSS25 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human HTRA2/Omi. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:160, 000 (at least detecting 6.25ng/ml)) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Apoptosis, Autophagy |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
HTRA2 also called Omi is a mammalian serine protease at high temperatures and has a chaperone activity at low temperature. The full-length HTRA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HTRA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HTRA2 has known to contribute both to caspase-dependent and caspase-independent cell death.
Defects in HTRA2 are the cause of Parkinson disease type 13. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
Defects in HTRA2 are the cause of Parkinson disease type 13. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat HTRA2 has not yet been tested.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
Related Products
Rabbit Anti-Human HTRA2 (C-terminus)(cat# DS-PB-02627) Custom Human HTRA2 ELISA Kit,(cat#ELH-HTRA2-001) Recombinant Human HTRA2 (cat#228-10807-3)
References
- Strauss, K. M. et al. (2005) Loss of function mutations in the gene encoding Omi/HtrA2 in Parkinson's disease. Human Molecular Genetics 14:2099-2111.
- Suzuki, Y. et al. (2001) A Serine Protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell. 8:613-621.
Expiration:
12 months from the date of shipment when stored properly.