RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-Human CD133 Antibody, APC

Catalog #: 136-25095

Human CD133 Antibody [clone: HB#7], monoclonal mouse IgG1. Validated for flow cytometry.

Product Description

Specifications

Size25 tests, 100 tests
Estimated Lead Time1-2 business days
SpeciesHuman
Host SpeciesMouse
Accession Number
O43490
Gene Id
8842
Gene Symbols
PROM1, PROML1, MSTP061
Protein Name / Synonyms
Prominin-1 (Antigen AC133) (Prominin-like protein 1) (CD antigen CD133)
ClonalityMonoclonal
CloneHB#7
ConjugationAPC
PreparationThe antibody was purified by affinity chromatography.
IsotypeIgG1
Format
Liquid
Concentration (lot specific)
5 μl/test
BuffersPBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide
Recommended ApplicationsFlow Cytometry
Research AreaCardiovascular Disease, Wnt-beta-Catenin Signaling
Excitation LaserRed Laser, 633 nm
Maximum Emission Wavelength
676 nm
Shipping TypeBlue ice
Storage4°C

Description

Product Features

  • Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
  • Gentle elution at pH 4.0 to minimize antibody degradation
  • Protein A, G & L affinity purification
  • High purity

Description

CD133 is a 150kD pentaspan glycoprotein with 5 transmembrane domains. It is a surface antigen for several different types of stem cells including endothelial progenitor cells, hematopoietic stem cells, glioblastomas, and glial stem cells. CD133 is found on both normal tissue as well as cancerous cells including some leukemia cells and brain tumor cells. CD133 is best understood to be a stem cell marker for both normal and cancerous cells.

Recommended Usage

Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.

Storage / Stability

Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.