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Anti-Human CD133 Antibody, Purified
Catalog #: 136-00095
Human CD133 Antibody [clone: HB#7], monoclonal Mouse IgG1. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | O54990 |
| Gene Symbols | CD133 |
| Protein Name / Synonyms | CD133, Prominin-1, PROM1, AC133 |
| Clonality | Monoclonal |
| Clone | HB#7 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Description
CD133 is a 150kD pentaspan glycoprotein with 5 transmembrane domains. It is a surface antigen for several different types of stem cells including endothelial progenitor cells, hematopoietic stem cells, glioblastomas, and glial stem cells. CD133 is found on both normal tissue as well as cancerous cells including some leukemia cells and brain tumor cells. CD133 is best understood to be a stem cell marker for both normal and cancerous cells.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.