3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Human CD133 Antibody, RayBright® Red 647
Catalog #: 136-24095
Human CD133 Antibody [clone: HB#7], monoclonal mouse IgG1. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | O43490 |
| Gene Id | 8842 |
| Gene Symbols | PROM1, PROML1, MSTP061 |
| Protein Name / Synonyms | Prominin-1 (Antigen AC133) (Prominin-like protein 1) (CD antigen CD133) |
| Clonality | Monoclonal |
| Clone | HB#7 |
| Conjugation | RayBright® Red 647 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 664 nm, replace Cy5™ and Alexa Fluor™ 647 (Cy5™ and Alexa Fluor™ are the trademark of Invitrogen and GE Health Care) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Human PBMCs were stained with RayBright® Red 647 Anti-Human CD133 antibody (#136-24095, left), or mouse IgG isotype control (right).
Description
CD133 is a 150kD pentaspan glycoprotein with 5 transmembrane domains. It is a surface antigen for several different types of stem cells including endothelial progenitor cells, hematopoietic stem cells, glioblastomas, and glial stem cells. CD133 is found on both normal tissue as well as cancerous cells including some leukemia cells and brain tumor cells. CD133 is best understood to be a stem cell marker for both normal and cancerous cells.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.