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Anti-Human CD154 (CD40L) Antibody, Purified
Catalog #: 136-00036
Human CD154 (CD40L) Antibody [clone: 5c8], monoclonal Mouse IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | CD40L |
| Clonality | Monoclonal |
| Clone | 5c8 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a Kappa |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Typical Data
PHA stimulated (3 days) human PBMCs were stained with either Purified Mouse Anti-Human CD40L primary antibody (#136-00036, blue histogram), or mouse IgG isotype control (gray histogram), followed by anti-mouse AF647 IgG/IgM secondary antibody.
Description
CD154 is ligand of CD40, also named as CD40L, is a 32-39 kD type II transmembrane glycoprotein and a member of the TNF superfamily mainly expressed on activated T cells. CD40L binds CD40 on antigen presenting cells and enhances their activation, maturation and role for co-stimulation. CD40L is particularly important on a subset of T cells called T follicular helper cells (TFH cells). CD40L on TFH cell binds CD40 on B cell to induce its differentiation, isotype class-switching and protects B cells from apoptosis.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.