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Anti-Human CD20 Antibody, RayBright® Red 647
Catalog #: 136-24045
Human CD20 Antibody [clone: 1F5], monoclonal mouse IgG2a. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | CD20, Bp35 |
| Clonality | Monoclonal |
| Clone | 1F5 |
| Conjugation | RayBright® Red 647 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 664 nm, replace Cy5™ and Alexa Fluor™ 647 (Cy5™ and Alexa Fluor™ are the trademark of Invitrogen and GE Health Care) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Human PBMCs were stained with RayBright® Red 647 Anti-Human CD20 (#136-24045, solid line), or mouse IgG isotype control (dashed line). CD3 negative population was gated.
Description
CD20 is a four transmembrane spanning protein and is expressed on pre-B-cells, resting and activated B cells, and some follicular dentritic cells. CD20 is involved in B cell activation and is associated with Src family kinases.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.