3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Human CD3 Antibody, Biotinylated
Catalog #: 136-01012
Biotinylated Human CD3 Antibody [clone: OKT3], monoclonal Mouse IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Gene Symbols | CD3 |
| Clonality | Monoclonal |
| Clone | OKT3 |
| Conjugation | Biotin |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin |
| Isotype | IgG2a Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Human PBMCs were stained with either Biotin Mouse Anti-Human CD3 antibody (#136-01012, solid histogram), or Purified Mouse Anti-Human CD3 antibody (#136-00012, dashed histogram), followed by PE-streptavidin.
Description
CD3ε, a subunit of CD3, is a 20 kDa transmembrane protein primarily expressed on pre-mature thymocytes, peripheral mature T cells, and NK-T cells. The CD3 molecules consist of CD3ε, δ, γ, and ζ chains and form a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The TCR on T cell surface is non-covalently associated with CD3 subunits CD3εγ and CD3εδ heterodimers and the CD3ζζ homodimer, which collectively form the TCR-CD3 complex. Antigen presentation of peptide-loaded MHC molecules by APCs, along with other co-stimulation signals, leads to activation of T cells. This antibody can recognize human CD3ε. It can also activate T cells in vitro together with anti-human CD28.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Stored at 2 °C - 8 °C, protected from prolonged light exposure. DO NOT FREEZE.