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Anti-Human CD32 Antibody, Purified
Catalog #: 136-00068
Human CD32 Antibody [clone: IV.3], monoclonal Mouse IgG2b. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Clonality | Monoclonal |
| Clone | IV.3 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2b |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Typical Data
Human PBMCs were stained with either Purified Mouse Anti-Human CD32 primary antibody (#136-00068, left), or mouse IgG isotype control (right), followed by anti-mouse IgG/IgM RayBright R 647 secondary antibody.
Description
CD32 is a polymorphic transmembrane glycoprotein in the immunoglobulin superfamily and is expressed on monocytes, granulocytes, platelets and B cells. CD32 has an affinity to aggregated IgG and immune complexes and has a role in regulating inflammation and immunity.