3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Human CD4 Antibody, PE
Catalog #: 136-18014
PE Anti-Human CD4 Antibody [clone: OKT4], monoclonal Mouse IgG2b kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Gene Symbols | CD4 |
| Protein Name / Synonyms | CD4 |
| Clonality | Monoclonal |
| Clone | OKT4 |
| Conjugation | PE |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with a high quality fluorochrome, PE |
| Isotype | IgG2b Kappa |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Blue Laser, 488 nm / Green Laser, 561 nm |
| Maximum Emission Wavelength | 575 nm |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Description
CD4 (cluster of differentiation 4), also known as T4/Leu-3, is a 55 kDa glycoprotein. It is a member of the Ig superfamily, primarily expressed on T helper cells, monocytes, macrophages, dendritic cells and some thymocytes (CD4+CD8+ and CD4+CD8 thymocytes). CD4 acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. HIV-1 gp120 protein binds CD4 to enter host T-cells, allowing HIV-1 to bind a co-receptor CCR5 or CXCR4 and fuse the outer membrane of the virus with the host cell membrane.
Product Details
Other Names
cluster of differentiation 4, T4, Leu-3
Recommended Usage
Each lot of antibody has been pre-titrated and tested by flow cytometry staining. We suggest use 5 µl per 106 cells in 100 µl volume of staining buffer.
Storage/Stability
Stored at 2°C ~ 8°C, protected from prolonged light exposure. DO NOT FREEZE.