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Anti-Human CD4 Antibody, PerCP
Catalog #: 136-16014
Human CD4 Antibody [clone: OKT4], monoclonal mouse IgG2b kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Gene Symbols | CD4 |
| Protein Name / Synonyms | CD4, cluster of differentiation 4, T4, Leu-3 |
| Clonality | Monoclonal |
| Clone | OKT4 |
| Conjugation | PerCP |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2b Kappa |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Blue Laser, 488 nm |
| Maximum Emission Wavelength | 675 nm |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD4 (cluster of differentiation 4), also known as T4/Leu-3, is a 55 kDa glycoprotein. It is a member of the Ig superfamily, primarily expressed on T helper cells, monocytes, macrophages, dendritic cells and some thymocytes (CD4+CD8+ and CD4+CD8– thymocytes). CD4 acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. HIV-1 gp120 protein binds CD4 to enter host T-cells, allowing HIV-1 to bind a co-receptor CCR5 or CXCR4 and fuse the outer membrane of the virus with the host cell membrane.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.