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Anti-Human CD4 Antibody, Purified
Catalog #: 136-00014
Human CD4 Antibody [clone: OKT4], monoclonal Mouse IgG2b kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Gene Symbols | CD4 |
| Protein Name / Synonyms | cluster of differentiation 4, T4, Leu-3 |
| Clonality | Monoclonal |
| Clone | OKT4 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2b Kappa |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Typical Data
Human PBMCs were stained with either Purified Mouse Anti-Human CD4 primary antibody (#136-00014, blue histogram), or mouse IgG isotype control (gray histogram), followed by AF 647 anti-mouse IgG/IgM secondary antibody.
Description
CD4 (cluster of differentiation 4), also known as T4/Leu-3, is a 55 kDa glycoprotein. It is a member of the Ig superfamily, primarily expressed on T helper cells, monocytes, macrophages, dendritic cells and some thymocytes (CD4+CD8+ and CD4+CD8‚¬€œ thymocytes). CD4 acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. HIV-1 gp120 protein binds CD4 to enter host T-cells, allowing HIV-1 to bind a co-receptor CCR5 or CXCR4 and fuse the outer membrane of the virus with the host cell membrane.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.