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Anti-Human CD40 Antibody, RayBright® Violet 450
Catalog #: 136-08042
Human CD40 Antibody [clone: G28-5], monoclonal mouse IgG1 kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P25942 |
| Gene Id | 958 |
| Gene Symbols | CD40, TNFRSF5 |
| Protein Name / Synonyms | Tumor necrosis factor receptor superfamily member 5 (B-cell surface antigen CD40) (Bp50) (CD40L receptor) (CDw40) (CD antigen CD40) |
| Clonality | Monoclonal |
| Clone | G28-5 |
| Conjugation | RayBright® Violet 450 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 Kappa |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Angiogenesis, Cardiovascular Disease, Cancer, Inflammation |
| Excitation Laser | Violet Laser, 407 nm |
| Maximum Emission Wavelength | 454 nm, replace Pacific Blue™ |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD40 (cluster of differentiation 40), a 48 kD type I glycoprotein, is a member of the TNFR superfamily, a costimulatory factor found on antigen presenting cells and is required for their activation. CD40 is primarily expressed on surface of dendritic cells, B cells, and macrophages and on endothelial, smooth muscle cells, and fibroblasts. Binding of CD40 to CD154 (CD40L) on T helper cells activates antigen presenting cells and enhances the expression of cytokines, chemokines, matrix metalloproteinases, growth factors, and adhesion molecules. CD40L on T cell also binds CD40 on B cell to induce its differentiation, isotype class-switching and protects B cells from apoptosis.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.