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Anti-Human CD44 Antibody, Biotinylated
Catalog #: 136-01016
Biotinylated Human CD44 Antibody [clone: FW11-10-03], monoclonal Mouse IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Gene Symbols | CD44 |
| Clonality | Monoclonal |
| Clone | FW11-10-03 |
| Conjugation | Biotin |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin |
| Isotype | IgG2a Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD44 is an 80-95 kDa glycoprotein expressed by a variety of hematopoietic and non-hematopoietic cells including all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. CD44 combined with CD62L, also called L-selectin, has been used as a T and B cell activation marker. CD44 expression is upregulated in naive T cells upon activation, and high CD44 expression is maintained on memory T cells. Therefore, CD44 is often used to discriminate CD44hi antigen€“experienced T cells from CD44low (CD62Lhi) naive T cells. CD44 is also an adhesion molecule involved in leukocyte attachment to endothelial cells, homing to peripheral lymphoid organs and sites of inflammation, and aggregating leukocytes.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Stored at 2 °C - 8 °C, protected from prolonged light exposure. DO NOT FREEZE.