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Anti-Human CD45 Antibody, Biotinylated
Catalog #: 136-01017
Biotinylated Human CD45 Antibody [clone: 2B2-A11-C5], monoclonal Mouse IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Gene Symbols | CD45 |
| Protein Name / Synonyms | protein tyrosine phosphatase receptor type C (PTPRC) |
| Clonality | Monoclonal |
| Clone | 2B2-A11-C5 |
| Conjugation | Biotin |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin |
| Isotype | IgG2a Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD45, also known as protein tyrosine phosphatase receptor type C (PTPRC), is a 180 - 240 kDa single chain type I membrane glycoprotein. CD45 is expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets, making it also known as leukocyte common antigen (LCA). CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 has various isoforms based on gene splicing of its three exons RA, RB and RC: CD45RA, CD45RB, CD45RC, CD45RAB, CD45RAC, CD45RBC, CD45R0, CD45R (ABC). This antibody directs to a reserved region, recognizes all different isoforms of CD45 and is often used to discriminate leukocytes from other cell types.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Stored at 2 °C - 8 °C, protected from prolonged light exposure. DO NOT FREEZE.