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Anti-Human CD56 Antibody, Biotinylated
Catalog #: 136-01047
Biotinylated Human CD56 Antibody [clone: 5.1H], monoclonal Mouse IgG1 kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | NCAM |
| Clonality | Monoclonal |
| Clone | 5.1H |
| Conjugation | Biotin |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Human PBMCs were stained with either Biotin Mouse Anti-Human CD56 antibody (#136-01047, solid histogram), or Purified Mouse Anti-Human CD56 antibody (#136-00047, dashed histogram), followed by PE-streptavidin.
Description
CD56 is a member of the Ig superfamily. The ~140 kDa isoform of CD56 is considered the prototypic cell surface marker on human NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions, certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias. CD56 mediates cell-cell and cell-matrix adhesion via homophilic and heterophilic interactions, respectively, and plays a role in activating intracellular signaling pathways.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Stored at 2 °C - 8 °C, protected from prolonged light exposure. DO NOT FREEZE.