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Anti-Human CD62L Antibody, RayBright® Red 647
Catalog #: 136-24038
Human CD62L Antibody [clone: DREG56], monoclonal mouse IgG1 kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P14151 |
| Gene Id | 6402 |
| Gene Symbols | SELL, LNHR, LYAM1 |
| Protein Name / Synonyms | L-selectin (CD62 antigen-like family member L) (Leukocyte adhesion molecule 1) (LAM-1) (Leukocyte surface antigen Leu-8) (Leukocyte-endothelial cell adhesion molecule 1) (LECAM1) (Lymph node homing receptor) (TQ1) (gp90-MEL) (CD antigen CD62L) |
| Clonality | Monoclonal |
| Clone | DREG56 |
| Conjugation | RayBright® Red 647 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 Kappa |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 664 nm, replace Cy5™ and Alexa Fluor™ 647 (Cy5™ and Alexa Fluor™ are the trademark of Invitrogen and GE Health Care) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Human PBMCs were stained with RayBright® Red 647 Anti-Human CD62L antibody (#136-24038, solid line), or mouse IgG isotype control (dashed line).
Description
CD62L antigen, a 74 kDa glycoprotein and a member of the selectin family, also known as L-selectin, LECAM-1, or LAM-1, is a cell adhesion molecule found on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds a series of glycoproteins including CD34, GlyCAM-1, and MAdCAM-1 and is important for homing of naive lymphocytes via endothelial venules to peripheral lymph nodes and Peyer’s patches. The CD62L antigen also contributes to the recruitment of leukocytes from the blood to areas of inflammation. Naive T cells and central memory T cells express high level of CD62L. CD62L is downregulated in activated T cells and effector memory T cells. High expression of CD62L on human bone marrow progenitor cells is an early sign of cells becoming committed to lymphoid differentiation.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.