Human CD90 Antibody [clone: K117], monoclonal mouse IgG1 kappa. Validated for flow cytometry.
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Product Description
Specifications
| Size | 25 tests, 100 tests |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P04216 |
| Gene Id | 7070 |
| Gene Symbols | THY1 |
| Protein Name / Synonyms | Thy-1 membrane glycoprotein (CDw90) (Thy-1 antigen) (CD antigen CD90) |
| Clonality | Monoclonal |
| Clone | K117 |
| Conjugation | RayBright® Blue 488 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 Kappa |
| Format | Liquid |
| Concentration (lot specific) | 5 μl/test |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Excitation Laser | Blue Laser, 488 nm |
| Maximum Emission Wavelength | 518 nm, replace FITC, Alexa Fluor™ 488, and BD BB515 (Alexa Fluor™ is the trademark of Invitrogen) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Human MSCs were stained with RayBright® Blue 488 Anti-Human CD90 antibody (#136-14048, solid line), or mouse IgG isotype control (dashed line).
Description
CD90, a 25-35 kD GPI-anchored protein also named Thy-1, is a member of the Ig superfamily. Human CD90 is expressed on neuronal cells and human thymocytes, a subset of CD34+ cells, a subset of fetal liver cells, fibroblasts, activated endothelial cells, and some leukemia cell lines. Hematopoietic Stem Cells (HSC) express both CD34 and CD90. CD90 plays roles in signal transduction, cell adhesion and migration, neurite outgrowth, T cell activation, tumor suppression, and inhibition of the proliferation and differentiation of hematopoietic stem cells.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.
