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Anti-Human CD95 Antibody, Biotinylated
Catalog #: 136-01073
Biotinylated Human CD95 Antibody [clone: huFasM38], monoclonal Mouse IgG1. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Protein Name / Synonyms | Fas antigen, APO-1, TNFRSF6 |
| Clonality | Monoclonal |
| Clone | huFasM38 |
| Conjugation | Biotin |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD95 is a single chain type I glycoprotein in the TNF receptor superfamily which is expresed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. When activation takes place, CD95 is upregulated. When the extracellular region of CD95 binds to the Fas ligand (CD178) apoptosis is induced.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Stored at 2 °C - 8 °C, protected from prolonged light exposure. DO NOT FREEZE.