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Anti-HYAL1 Antibody
Catalog #: 144-06623
HYAL1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q12794 |
| Gene Id | 3373 |
| Gene Symbols | HYAL1 |
| Protein Name / Synonyms | HYAL1, HYAL-1, LUCA1, MPS9, NAT6, hyaluronidase-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
10kDa/19kDa/23kDa/27kDa/37kDa/45kDa/48kDa
Observed MW
48kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 136-435 of human HYAL1 (NP_149349.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HYAL1 Antibody (144-06623) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver injury using HYAL1 antibody (144-06623) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HYAL1 antibody (144-06623) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HYAL1 antibody (144-06623) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HYAL1 antibody (144-06623) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.