RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-ICAM1 Antibody

Catalog #: 144-60414

ICAM1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P05362
Gene Id
3383
Gene Symbols
ICAM1
Protein Name / Synonyms
ICAM1, BB2, CD54, P3.58, intercellular adhesion molecule 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
57kDa
Observed MW
80kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 250-480 of human ICAM1 (NP_000192.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ICAM1 antibody (144-60414) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - ICAM1 antibody (144-60414)

Expiration:
12 months from the date of shipment when stored properly.