Anti-ICOS Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9Y6W8
Gene Id	29851
Gene Symbols	ICOS
Protein Name / Synonyms	ICOS, AILIM, CD278, CVID1, inducible T-cell costimulator
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

19kDa/22kDa

Observed MW

26kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-140 of human ICOS (NP_036224.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ICOS antibody (144-01811) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - ICOS antibody (144-01811)

Immunohistochemistry of paraffin-embedded mouse spleen using ICOS antibody (144-01811) at dilution of 1:100 (40x lens).
Immunohistochemistry - ICOS antibody (144-01811)

Immunofluorescence analysis of MCF-7 cells using ICOS antibody (144-01811). Blue: DAPI for nuclear staining.
Immunofluorescence - ICOS antibody (144-01811)

Expiration:

12 months from the date of shipment when stored properly.

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