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Anti-IFI16 Antibody
Catalog #: 144-60546
IFI16 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16666 |
| Gene Id | 3428 |
| Gene Symbols | IFI16 |
| Protein Name / Synonyms | IFI16, IFNGIP1, PYHIN2, gamma-interferon-inducible protein 16 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
75kDa/82kDa/88kDa
Observed MW
88kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human IFI16 (NP_005522.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IFI16 antibody (144-60546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat intestine using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.