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Anti-IGF-1 Antibody
Catalog #: 130-10063
Mouse Anti-Human IGF-1 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein derived from IGF-1. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 6.25 ng/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, 3T3-L-1 lysate. The membrane blot was probed with anti-IGF-1 primary antibody (1 µg/ ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system. |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | IGF Signaling |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Human Long-[arg3] Insulin like Growth Factor-1 (IGF-1), also called as IGF long R3 protein, mediates many of the growth-promoting effects of growth hormone. IGF1 also stimulates both proliferation and differentiation of myogenic precursor cells. In vivo IGFs are bound to one of the members of a family of six high-affinity IGF binding proteins (IGFBP 1-6) that regulate their biological activity. One of these binding proteins, IGFBP-3, affects cell proliferation via both IGF-dependent and IGF-independent mechanisms and it has generally been shown to suppress proliferation of cultured cells.
Specificity
The Mouse anti-human IGF-1 antibody specifically detects targets derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilizedantibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
References
- Laajoki LG, et al. (2000) Solution structure and backbone dynamics of long-[Arg(3)]insulin-like growth factor-I. J Biol Chem. 7; 275(14):10009-15.
- Richards RG, et al. (1998) Insulin-like growth factor-1 (IGF-1) receptor-insulin receptor substratecomplexes in the uterus. Altered signaling response to estradiol in the IGF-1(m/m) mouse. J Biol Chem. 8;273(19):11962-9.
- Valverde AM, et al. (1998) Insulin receptor substrate (IRS) proteins IRS-1 and IRS-2 differential signaling in the insulin/insulin-like growth factor-I pathways in fetal brown adipocytes. Mol Endocrinol. 12(5):688-97.
Expiration:
12 months from the date of shipment when stored properly.