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Anti-IGF-1 Antibody, Biotinylated
Catalog #: 130-10063B-50
Mouse anti-IGF-1 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein derived from IGF-1. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Isotype | IgG1 |
| Recommended Applications | ELISA (recommended work dilution= 6.25 µg/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, 3T3-L-1 lysate. The membrane blot was probed with anti-IGF-1 primary antibody (1 µg/ ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system. |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | IGF Signaling |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Human Long-[arg3] Insulin like Growth Factor-1 (IGF-1), also called as IGF long R3 protein, mediates many of the growth-promoting effects of growth hormone. IGF1 also stimulates both proliferation and differentiation of myogenic precursor cells. In vivo IGFs are bound to one of the members of a family of six high-affinity IGF binding proteins (IGFBP 1-6) that regulate their biological activity. One of these binding proteins, IGFBP-3, affects cell proliferation via both IGF-dependent and IGF-independent mechanisms and it has generally been shown to suppress proliferation of cultured cells.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The Mouse anti-human IGF-1 antibody specifically detects targets derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
References
- Laajoki LG, et al. (2000) Solution structure and backbone dynamics of long-[Arg(3)]insulin-like growth factor-I. J Biol Chem. 7; 275(14):10009-15.
- Richards RG, et al. (1998) Insulin-like growth factor-1 (IGF-1) receptor-insulin receptor substratecomplexes in the uterus. Altered signaling response to estradiol in the IGF-1(m/m) mouse. J Biol Chem. 8;273(19):11962-9.
- Valverde AM, et al. (1998) Insulin receptor substrate (IRS) proteins IRS-1 and IRS-2 differential signaling in the insulin/insulin-like growth factor-I pathways in fetal brown adipocytes. Mol Endocrinol. 12(5):688-97.
Expiration:
12 months from the date of shipment when stored properly.