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Anti-IGF1R Antibody
Catalog #: 144-00243
IGF1R Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P08069 |
| Gene Id | 3480 |
| Gene Symbols | IGF1R |
| Protein Name / Synonyms | IGF1R, CD221, IGFIR, IGFR, JTK13, insulin-like growth factor 1 receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 1.04 mg/ml |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
154kDa
Observed MW
127kDa
Immunogen
A synthetic peptide of human IGF1R
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IGF1R antibody (144-00243) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse liver using IGF1R antibody (144-00243) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IGF1R antibody (144-00243) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using IGF1R antibody (144-00243) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.