Anti-IGF2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P01344
Gene Id	3481
Gene Symbols	IGF2
Protein Name / Synonyms	IGF2, C11orf43, GRDF, IGF-II, PP9974, insulin like growth factor 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW

20kDa/26kDa

Observed MW

28kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 25-180 of human IGF2 (NP_000603.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using IGF2 antibody (144-02086) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - IGF2 Polyclonal Antibody (144-02086)

Immunofluorescence analysis of A-549 cells using IGF2 antibody (144-02086). Blue: DAPI for nuclear staining.
Immunofluorescence - IGF2 Polyclonal Antibody (144-02086)

Expiration:

12 months from the date of shipment when stored properly.

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