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Anti-IGF2BP1 Antibody
Catalog #: 144-01517
IGF2BP1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9NZI8 |
| Gene Id | 10642 |
| Gene Symbols | IGF2BP1 |
| Protein Name / Synonyms | IGF2BP1, CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1, insulin-like growth factor 2 mRNA-binding protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IHC 1:50 - 1:100
IF 1:50 - 1:200
Format
Calculated MW
48kDa/63kDa
Observed MW
71kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human IGF2BP1 (NP_006537.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IGF2BP1 antibody (144-01517) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using IGF2BP1 antibody (144-01517) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using IGF2BP1 antibody (144-01517) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.